General description

Glycerol, also referred to as glycerin or glycerine, is a 3 carbon sugar alcohol that forms the backbone of fatty acids. It is a central component for synthesis of all lipids, and acts as a backbone for triglycerides and phospholipids, which play an important role in cell membrane structure. Due to its low toxicity, glycerol is widely used in pharmaceutical, food and cosmetic formulations and is the main waste by-product of biodiesel production via transesterification

Principle

The High Sensitivity Free Glycerol Assay Kit is suitable for measuring trace amounts of glycerol in samples, which contain reducing substances that may interfere with oxidase-based assays. This assay employs coupled enzyme reaction, which results in a fluorometric product (lex = 535 nm/lem = 587 nm), proportional to the amount of glycerol present. This assay kit is simple and sensitive with the capability to detect ﹤40 pmole glycerol in a variety of samples.

Suitability

Suitable for use with serum, plasma, and other biological fluids, and tissue and cell culture samples.